EIDC
Erezyilmaz, D.; Doubovetzky, N.; Billeter, J.C.; Goodwin, S.F.

Levels of the pheromone 7,11-heptacosadiene in the sibling species Drosophila simulans and Drosophila sechellia

https://doi.org/10.5285/7abe787a-4561-4715-8b49-983faa4c0787 Cite this dataset
This dataset provides measurements of the cuticular pheromone, 7,11-heptacosadiene of individual female vinegar flies. The purpose of the dataset is to compare the levels between two sibling species, Drosophila simulans and Drosophila sechellia. Single female virgins were isolated from males at eclosion, aged five days, and then washed in hexane to extract cuticular hydrocarbons. The extracts were analyzed by gas chromatography relative to an internal standard.
Publication date: 2023-02-20

Format

Comma-separated values (CSV)

Provenance & quality

Drosophila females that were five days old were anaesthetised on ice for ten minutes. Individual female flies were then transferred to 150uL pure HPLC grade hexane within a 200uL glass vial that was fitted with a glass insert. The vial with the fly inside was vortexed to extract the hydrocarbons. Afterwards the fly was removed, the hexane extract was dried down under a gentle flow of nitrogen, and the vial was shipped to Groningen for gas chromatograph analysis. The retention time in minutes of each peak relative to an internal standard (C26), was used to measure size and identify 7,11-heptacosadiene. The area underneath each peak, relative to the standard was used to measure the quantity of 7,11-heptacosadiene. ChemStation software (Agilent technologies) was used to estimate area under the peak.

Correspondence/contact details

Erezyilmaz, D.
University of Oxford
 deniz.erezyilmaz@dpag.ox.ac.uk

Authors

Erezyilmaz, D.
University of Oxford
ORCID iD icon https://orcid.org/0000-0001-8416-8561
Doubovetzky, N.
University of Groningen
ORCID iD icon https://orcid.org/0000-0001-6219-3125
Billeter, J.C.
University of Groningen
ORCID iD icon https://orcid.org/0000-0001-7796-4068
Goodwin, S.F.
University of Oxford
ORCID iD icon https://orcid.org/0000-0002-0552-4140

Other contacts

Rights holder
University of Oxford
Custodian
NERC EDS Environmental Information Data Centre
 info@eidc.ac.uk
Publisher
NERC EDS Environmental Information Data Centre
 info@eidc.ac.uk

Additional metadata

Topic categories
biota
environment
Keywords
contact pheromone , Cuticular hydrocarbons , Drosophila courtship , gas chromatography , long-chain fatty acids
Funding
Natural Environment Research Council Award: NE/S010351/1
Last updated
27 February 2024 16:26

Get the data

By accessing or using this dataset, you agree to the terms of the relevant licence agreement(s). You will ensure that this dataset is cited in any publication that describes research in which the data have been used.

This dataset is available under the terms of the Open Government Licence

Download the data Supporting documentation

CITE AS: Erezyilmaz, D.; Doubovetzky, N.; Billeter, J.C.; Goodwin, S.F. (2023). Levels of the pheromone 7,11-heptacosadiene in the sibling species Drosophila simulans and Drosophila sechellia. NERC EDS Environmental Information Data Centre. https://doi.org/10.5285/7abe787a-4561-4715-8b49-983faa4c0787